UPS2
Sigma
Proteomics Dynamic Range Standard Set
Description
| Application | The Proteomics Dynamic Range Standard Set can be used to standardize and/or evaluate mass spectrometric (e.g., LC-MS/MS, MALDI-TOF-MS, etc.) and electrophoretic analysis conditions prior to the analysis of complex protein samples. UPS2 can be used to bracket precious experimental data sets between runs of a known complex standard sample. This allows confirmation of the robustness of the analysis method and stability of the instrument employed. Additionally, laboratories generating or comparing mass spectrometric data derived from poorly defined samples can use UPS2 as an external reference to assist with the evaluation of results and experimental methodology. |
| General description | The Proteomics Dynamic Range Standard Set is produced from a mixture of 48 individual human source or human sequence recombinant proteins, each of which has been selected to limit heterogeneous post-translational modifications (PTMs). The protein standard is formulated from 6 mixtures of 8 proteins to present a dynamic range of 5 orders of magnitude, ranging from 50 pmoles to 500 amoles. Each protein has been quantitated by amino acid analysis (AAA) prior to formulation. |
Properties
| concentration | 10.6 μg/ampule protein |
| shipped in | wet ice |
| storage temp. | −20°C |
Safety
| Hazard Codes | Xn,B |
| Risk Statements | 22-36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
Components
| Kit component only | Proteomics Dynamic Range Standard 1 vial |
| Kit component also available separately | Trypsin from porcine pancreas, Proteomics Grade, Dimethylated 20 μg T6567 |
Related Products
| Related product | UPS1, Universal Proteomics Standard Set |
References
| reference | Tabb, D.L., et al., MyriMatch: highly accurate tandem mass spectral peptide identification by multivariate hypergeometric analysis. J. Proteome Res. 6, 654-661, (2007) Abstract |
| Uwaje, N.C., et al., Interrogation of MS/MS search data with an pI Filter algorithm to increase protein identification success. Electrophoresis 28, 1867-1874, (2007) Abstract | |
| Nesvizhskii AI., Protein identification by tandem mass spectrometry and sequence database searching Methods Mol. Biol. 367, 87-119, (2007) Abstract |
